Characterization of Two Species of Methionine Transfer Ribonucleic Acid from Bakers' Yeast
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چکیده
منابع مشابه
Characterization of two species of methionine transfer ribonucleic acid from bakers' yeast.
The biochemical properties of two species of methionine transfer ribonucleic acid from bakers’ yeast, separated by diethylaminoethyl Sephadex column chromatography, have been studied. Each species of the two methionine tRNAs, designated methionine tRNA I and methionine tRNA II, respectively, has been purified by benzoylated DEAEcellulose column chromatography. Methionine tRNA I can be esterifie...
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Lysyl transfer ribonucleic acid synthetase (L-lysine: tRNA ligase (AMP), EC 6.1.1.6) was pursed to a state of apparent homogeneity from bakers’ yeast. For each of two different preparation procedures, a 250to 500-fold purikation of the enzyme was obtained, and the final purification step yielded two distinct protein components with specific activity values of approximately 0.5 and 1.0 pmole of ...
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Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...
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We are attempting to determine the nature of specific RNA-protein interactions by study of tRNA charging by homologous and heterologous enzymes. tRNAklatRNARNA3 t were prepared by phenoxyacetylation of alanyl-tRNA and separation on benzoylated DEAE-cellulose (Gillam, Blew, Warrington, von Tigerstrom & Tener, 1968). A partially purified alanyl-tRNA synthetase from yeast forms alanyl-tRNAAla tand...
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Fresh bakers’ yeast was obtained from the Red Star Company, Cleveland, Ohio. The procedure for the isolation of the RNA fractions and their designation were those of Crestfield et al. (3) and Davis and Allen (4). Briefly, 150-g batches of finely divided yeast were heated 1 minute at 83-87” and 2 minutes at 80” in a solution of 2% recrystallized Duponol C (sodium lauryl sulfate, U.S.P., du Pont)...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91930-7